tatAD [2018-06-22 18:47:40]

component of the TatAD-TatCD twin-arginine translocase

locus: BSU02630

pI: 9.69

mw: 7.37 kDa

protein length: 70 aa

gene length: 210 bp

function: TAT protein secretion

product: component of the twin-arginine translocation pathway

essential: no

synonyms: yczB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 285,775 → 285,987

The protein

Catalyzed reaction/ biological activity

• translocation of [protein|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|PhoD] [Pubmed|19395490]

Protein family

• tatA/E family (according to Swiss-Prot)

Structure

• [PDB|2L16] [Pubmed|20726548]

[SW|Localization]

• membrane (according to Swiss-Prot), forms foci at the division sites and cell poles, localization requires interaction with [protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|TatCD] or [protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY] [Pubmed|19395490]

Expression and Regulation

Operons

• genes: [gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10094677], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|10094677,11007775], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|10094677]

  additional information

  • expression of the operon depends on functional [protein|search|HemAT] and [protein|search|CsbC] [PubMed|23180473]

Biological materials

Mutant

• BKE02630 (Δ[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTGAAAACATAATTTCCA, downstream forward: _UP4_TGAATGCTGATGAGGCAGAC
• BKK02630 (Δ[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTGAAAACATAATTTCCA, downstream forward: _UP4_TGAATGCTGATGAGGCAGAC

Labs working on this gene/protein

• [SW|Oscar Kuipers], University of Groningen, The Netherlands
• [http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

• 22683878,24140208,25975269,25494301,27121927

Original publications

• 12867413,15554971,20726548,11007775,19395490,21479178,21479530,22383849,10094677,23180473,23567937,20977272,27984091